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Genetic deletion of Per1/Per2 enhances pulmonary leukocyte infiltration and lung tissue damage, which contribute to an early mortality in SCD. A) Schematic of SCD or WT BM transplanted to WT or Per1/Per2 dKO mice. B) Images of irradiated male mice ∼16 wk post-BMT. C) Increased mortality was identified beginning 42 d and up to 112 d after irradiation and hematopoietic adoptive transfer in female SCD BM transplanted to Per1/Per2 dKO mice. D) Panel of histologic stains, hematoxylin and eosin (H&E), and <t>Ly6-positive</t> leukocytes as indicated with red arrows in SCD or WT BM transplanted to WT or Per1/Per2 dKO mice. E) Semiquantification of histologic assessment of lung isolated from SCD or WT phenotypic mice with or without Per1/Per2. F) Quantification of leukocytes in ×20 field in whole lung sections isolated from SCD and WT BM transplant mice; n = 5 mice/group. ND, not determined. *P < 0.05, SCD → WT compared with WT → WT mice, **P < 0.01, SCD → WT compared with SCD → Per1/Per2 dKO mice.
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Genetic deletion of Per1/Per2 enhances pulmonary leukocyte infiltration and lung tissue damage, which contribute to an early mortality in SCD. A) Schematic of SCD or WT BM transplanted to WT or Per1/Per2 dKO mice. B) Images of irradiated male mice ∼16 wk post-BMT. C) Increased mortality was identified beginning 42 d and up to 112 d after irradiation and hematopoietic adoptive transfer in female SCD BM transplanted to Per1/Per2 dKO mice. D) Panel of histologic stains, hematoxylin and eosin (H&E), and <t>Ly6-positive</t> leukocytes as indicated with red arrows in SCD or WT BM transplanted to WT or Per1/Per2 dKO mice. E) Semiquantification of histologic assessment of lung isolated from SCD or WT phenotypic mice with or without Per1/Per2. F) Quantification of leukocytes in ×20 field in whole lung sections isolated from SCD and WT BM transplant mice; n = 5 mice/group. ND, not determined. *P < 0.05, SCD → WT compared with WT → WT mice, **P < 0.01, SCD → WT compared with SCD → Per1/Per2 dKO mice.
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Genetic deletion of Per1/Per2 enhances pulmonary leukocyte infiltration and lung tissue damage, which contribute to an early mortality in SCD. A) Schematic of SCD or WT BM transplanted to WT or Per1/Per2 dKO mice. B) Images of irradiated male mice ∼16 wk post-BMT. C) Increased mortality was identified beginning 42 d and up to 112 d after irradiation and hematopoietic adoptive transfer in female SCD BM transplanted to Per1/Per2 dKO mice. D) Panel of histologic stains, hematoxylin and eosin (H&E), and <t>Ly6-positive</t> leukocytes as indicated with red arrows in SCD or WT BM transplanted to WT or Per1/Per2 dKO mice. E) Semiquantification of histologic assessment of lung isolated from SCD or WT phenotypic mice with or without Per1/Per2. F) Quantification of leukocytes in ×20 field in whole lung sections isolated from SCD and WT BM transplant mice; n = 5 mice/group. ND, not determined. *P < 0.05, SCD → WT compared with WT → WT mice, **P < 0.01, SCD → WT compared with SCD → Per1/Per2 dKO mice.
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Oxford Biomedical Research monoclonal primary mouse anti-rat antibodies directed against cyp4502e1
Genetic deletion of Per1/Per2 enhances pulmonary leukocyte infiltration and lung tissue damage, which contribute to an early mortality in SCD. A) Schematic of SCD or WT BM transplanted to WT or Per1/Per2 dKO mice. B) Images of irradiated male mice ∼16 wk post-BMT. C) Increased mortality was identified beginning 42 d and up to 112 d after irradiation and hematopoietic adoptive transfer in female SCD BM transplanted to Per1/Per2 dKO mice. D) Panel of histologic stains, hematoxylin and eosin (H&E), and <t>Ly6-positive</t> leukocytes as indicated with red arrows in SCD or WT BM transplanted to WT or Per1/Per2 dKO mice. E) Semiquantification of histologic assessment of lung isolated from SCD or WT phenotypic mice with or without Per1/Per2. F) Quantification of leukocytes in ×20 field in whole lung sections isolated from SCD and WT BM transplant mice; n = 5 mice/group. ND, not determined. *P < 0.05, SCD → WT compared with WT → WT mice, **P < 0.01, SCD → WT compared with SCD → Per1/Per2 dKO mice.
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Becton Dickinson rat anti-mouse ac-3 monoclonal primary antibody (1:25 dilution, 550292, harmingen, san diego, ca)
Genetic deletion of Per1/Per2 enhances pulmonary leukocyte infiltration and lung tissue damage, which contribute to an early mortality in SCD. A) Schematic of SCD or WT BM transplanted to WT or Per1/Per2 dKO mice. B) Images of irradiated male mice ∼16 wk post-BMT. C) Increased mortality was identified beginning 42 d and up to 112 d after irradiation and hematopoietic adoptive transfer in female SCD BM transplanted to Per1/Per2 dKO mice. D) Panel of histologic stains, hematoxylin and eosin (H&E), and <t>Ly6-positive</t> leukocytes as indicated with red arrows in SCD or WT BM transplanted to WT or Per1/Per2 dKO mice. E) Semiquantification of histologic assessment of lung isolated from SCD or WT phenotypic mice with or without Per1/Per2. F) Quantification of leukocytes in ×20 field in whole lung sections isolated from SCD and WT BM transplant mice; n = 5 mice/group. ND, not determined. *P < 0.05, SCD → WT compared with WT → WT mice, **P < 0.01, SCD → WT compared with SCD → Per1/Per2 dKO mice.
Rat Anti Mouse Ac 3 Monoclonal Primary Antibody (1:25 Dilution, 550292, Harmingen, San Diego, Ca), supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Genetic deletion of Per1/Per2 enhances pulmonary leukocyte infiltration and lung tissue damage, which contribute to an early mortality in SCD. A) Schematic of SCD or WT BM transplanted to WT or Per1/Per2 dKO mice. B) Images of irradiated male mice ∼16 wk post-BMT. C) Increased mortality was identified beginning 42 d and up to 112 d after irradiation and hematopoietic adoptive transfer in female SCD BM transplanted to Per1/Per2 dKO mice. D) Panel of histologic stains, hematoxylin and eosin (H&E), and Ly6-positive leukocytes as indicated with red arrows in SCD or WT BM transplanted to WT or Per1/Per2 dKO mice. E) Semiquantification of histologic assessment of lung isolated from SCD or WT phenotypic mice with or without Per1/Per2. F) Quantification of leukocytes in ×20 field in whole lung sections isolated from SCD and WT BM transplant mice; n = 5 mice/group. ND, not determined. *P < 0.05, SCD → WT compared with WT → WT mice, **P < 0.01, SCD → WT compared with SCD → Per1/Per2 dKO mice.

Journal: The FASEB Journal

Article Title: Circadian period 2: a missing beneficial factor in sickle cell disease by lowering pulmonary inflammation, iron overload, and mortality

doi: 10.1096/fj.201900246RR

Figure Lengend Snippet: Genetic deletion of Per1/Per2 enhances pulmonary leukocyte infiltration and lung tissue damage, which contribute to an early mortality in SCD. A) Schematic of SCD or WT BM transplanted to WT or Per1/Per2 dKO mice. B) Images of irradiated male mice ∼16 wk post-BMT. C) Increased mortality was identified beginning 42 d and up to 112 d after irradiation and hematopoietic adoptive transfer in female SCD BM transplanted to Per1/Per2 dKO mice. D) Panel of histologic stains, hematoxylin and eosin (H&E), and Ly6-positive leukocytes as indicated with red arrows in SCD or WT BM transplanted to WT or Per1/Per2 dKO mice. E) Semiquantification of histologic assessment of lung isolated from SCD or WT phenotypic mice with or without Per1/Per2. F) Quantification of leukocytes in ×20 field in whole lung sections isolated from SCD and WT BM transplant mice; n = 5 mice/group. ND, not determined. *P < 0.05, SCD → WT compared with WT → WT mice, **P < 0.01, SCD → WT compared with SCD → Per1/Per2 dKO mice.

Article Snippet: Sections were incubated with Ly6 primary antibody (rat monoclonal anti-mouse, 1:500 dilution in blocking solution; BD Biosciences, San Jose, CA, USA) overnight at 4°C.

Techniques: Irradiation, Adoptive Transfer Assay, Isolation